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Anti -SAE1 Rabbit pAb

價格: 在線咨詢
貨號:
KT011944
產品規(guī)格:
100ul
Applications:
WB|IHC/IF
Species:
H/M/R,H/M/R
購買數(shù)量:
立即詢價 在線咨詢 400-1016-218
產品詳情 操作說明 注意事項

Product Information


Description

SAE1 rabbit polyclonal antibody

Protein full name

SUMO-activating enzyme subunit 1

Synonyms

Ubiquitin-like 1-activating enzyme E1A, Sae1, SUMO-activating enzyme subunit 1, Uble1a, Aos1, Sua1, N-terminally processed, Ubl1a1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse SAE1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

39 kDa

Observed MW.

39 kDa

Uniprot ID

Q9UBE0, Q9R1T2, Q6AXQ0




Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 500-1: 1000

testis

IHC/IF

Human, Mouse, Rat

1: 750-1: 3000

brain, breast cancer, colon, colon cancer, lung, small intestine, testis




Background


SAE1(SUMO-activating enzyme subunit 1) is also named as AOS1, SUA1, UBLE1A and belongs to the ubiquitin-activating E1 family. SAE1 and UBA2 form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2.




Images


Picture 1.png

Western blot analysis of SAE1 (GB111191) at dilution of 1: 500

Picture 2.png

Immunohistochemistry of paraffin embedded human breast cancer using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)

Picture 3.png

Immunohistochemistry of paraffin embedded human colon cancer using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)

Picture 4.png

Immunohistochemistry of paraffin embedded human colon using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)

Picture 5.png

Immunohistochemistry of paraffin embedded mouse brain using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)

Picture 6.png

Immunohistochemistry of paraffin embedded mouse small intestine using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)

Picture 7.png

Immunohistochemistry of paraffin embedded rat brain using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)


Picture 8.png

Immunohistochemistry of paraffin embedded rat colon using SAE1 (GB111191) at dilution of 1: 1200 (400x lens)

Picture 9.png

Immunofluorescence of paraffin embedded human breast cancer using SAE1 (GB111191) at dilution of 1: 1100 (400x lens)

Picture 10.png

Immunofluorescence of paraffin embedded mouse brain using SAE1 (GB111191) at dilution of 1: 1100 (400x lens)

Picture 11.png

Immunofluorescence of paraffin embedded mouse lung using SAE1 (GB111191) at dilution of 1: 1100 (400x lens)

Picture 12.png

Immunofluorescence of paraffin embedded rat brain using SAE1 (GB111191) at dilution of 1: 1100 (400x lens)

Picture 13.png

Immunofluorescence of paraffin embedded rat testis using SAE1 (GB111191) at dilution of 1: 1100 (300x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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