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Anti -Cyclin E1 Rabbit pAb

價(jià)格: 在線咨詢
貨號(hào):
KT011860
產(chǎn)品規(guī)格:
100 μL
Applications:
WB | IHC
Species:
H,M,R | H,M,R
購(gòu)買(mǎi)數(shù)量:
產(chǎn)品詳情 操作說(shuō)明 注意事項(xiàng)

Product Information


Description

Cyclin E1 rabbit polyclonal

Protein full name

G1/S-specific cyclin-E1

Synonyms

CCNE1, CCNE, pcyclin E1, Cyclin E1

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse Cyclin E1

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

47 kDa

Observed MW.

47 kDa

Uniprot ID

P24864, Q61457




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 200-1: 400

testis, colon, placenta

IHC

Human, Mouse, Rat

1: 100-1: 400

placenta, colon, testis




Background


The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK2, whose activity is required for cell cycle G1/S transition. This protein accumulates at the G1-S phase boundary and is degraded as cells progress through S phase. Overexpression of this gene has been observed in many tumors, which results in chromosome instability, and thus may contribute to tumorigenesis.




Images


Western blot analysis of Cyclin E1 (GB111936)

at dilution of 1: 400

Lane 1: HeLa cell lysate

Lane 2: MCF7 cell lysate

Lane 3: K562 cell lysate

Lane 4: Mouse testis tissue lysate

Lane 5: Mouse colon tissue lysate

Lane 6: Rat testis tissue lysate

Lane 7: Rat placenta tissue lysate

Immunohistochemical analysis of hyperbaric repair of paraffin-embedded human placenta using 

Cyclin E1 (GB111936) at dilution of 1: 200

Immunohistochemical analysis of hyperbaric repair of paraffin-embedded human colon using 

Cyclin E1(GB111936) at dilution of 1: 200

Immunohistochemical analysis of hyperbaric repair of paraffin-embedded mouse testis using 

Cyclin E1(GB111936) at dilution of 1: 200




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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