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Anti -Securin Rabbit pAb

價格: 在線咨詢
貨號:
KT011701
產(chǎn)品規(guī)格:
Applications:
WB | IHC | IF
Species:
M,R | H,M,R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

Securin rabbit polyclonal antibody

Protein full name

Securin

Synonyms

Pituitary tumor-transforming gene 1 protein, Pttg1, Pttg, Cut2, EAP1, ESP1, TUTR1

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Securin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus, Cytoplasm

Predicted MW.

22 kDa

Observed MW.

26 kDa

Uniprot ID

O95997, Q9CQJ7, P97613




Applications


Applications

Species

Dilution

Positive Sample

WB

Mouse, Rat

1: 500-1: 1000

testis, cecum, skin, epityphlon

IHC/IF

Human, Mouse, Rat

1: 500-1: 2800

placenta, testis




Background


PTTG1 is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The protein has transforming activity in vitro and tumorigenic activity in vivo, and is highly expressed in various tumors. This protein contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the protein can act as a transactivation domain. It is mainly a cytosolic protein, although it partially localizes in the nucleus.




Images


Picture 1.png

Western blot analysis of Securin (GB111047) at dilution of 1: 500

Picture 2.png

Immunohistochemistry of paraffin embedded mouse placenta using Securin (GB111047) at dilution of 1: 1400 (400x lens)

Picture 3.png

Immunohistochemistry of paraffin embedded rat testis using Securin (GB111047) at dilution of 1: 1400 (400x lens)




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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