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Anti -Villin Mouse mAb

價(jià)格: 在線咨詢
貨號(hào):
DRF101341
產(chǎn)品規(guī)格:
100 μL
Applications:
WB | IHC | IF
Species:
H,M,R | H,M,R | H,M,R
購買數(shù)量:
產(chǎn)品詳情 操作說明 注意事項(xiàng)

Product Information


Description

Villin mouse molyclonal

Protein full name

Villin-1

Synonyms

VIL1, VIL, Villin 1, D2S1471

Immunogen

Recombinant protein corresponding to

Mouse Villin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

93 kDa

Observed MW.

95 kDa

Uniprot ID

P09327, Q62468




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

small intestine, kidney, colon

IHC

Human, Mouse, Rat

1: 500-1: 2000

small intestine, jejunum

IF Human, Mouse, Rat 1: 500-1: 1000




Background


Villin 1 (VIL1) is a 95-kd F-actin bundling and severing protein and its expression is restricted to epithelial cells with a brush border, like epithelial cells of the intestinal mucosa, gall bladder, renal proximal tubules and ductuli efferentes of the testis. VIL1 has been reported to be an epithelial cell-specific anti-apoptotic protein, and to have an important function in regulating actin dynamics, cell morphology, epithelial-to-mesenchymal transitions, cell migration and cell survival. In addition, VIL1 is a useful diagnostic marker for of various cancer, like cervical and endometrial adenocarcinomas, renal cell carcinoma.




Images


Western blot analysis of Villin (GB121209)

at dilution of 1: 1000

Lane 1: CaCO2 cell lysate

Lane 2: Mouse small intestine tissue lysate

Lane 3: Mouse kidney tissue lysate

Lane 4: Mouse colon tissue lysate

Lane 5: Rat small intestine tissue lysate

Lane 6: Rat kidney tissue lysate

Lane 7: Rat colon tissue lysate

Immunohistochemistry analysis of paraffin-embedded human small intestine using Villin (GB121209) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded mouse jejunum using Villin (GB121209) at dilution of 1: 2000

Immunohistochemistry analysis of paraffin-embedded rat jejunum using

Villin (GB121209) at dilution of 1: 2000




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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