服務(wù)熱線
400-1016-218
聯(lián)系方式
- 手機(jī)：400-1016-218
- Q Q：355185756
- 郵箱：355185756@qq.com
- 地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室
Anti -RB-1 Rabbit pAb
價(jià)格：
在線咨詢
貨號(hào)：
DRF101235
產(chǎn)品規(guī)格：
100 μL
Applications：
IHC/IF
Species：
M/R,M/R
購買數(shù)量：
產(chǎn)品詳情
操作說明
注意事項(xiàng)
Product Information
|
Description |
RB-1 rabbit polyclonal |
|
Protein full name |
Retinoblastoma-associated protein |
|
Synonyms |
pRb, Rb, pp105, Rb1 |
|
Immunogen |
KLH conjugated Synthetic peptide corresponding to Mouse RB-1 |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Subcellular location |
|
|
Uniprot ID |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
IHC |
Mouse, Rat |
1: 700-1: 1400 |
brain, kidney, lung, skeletal muscle |
|
IF |
Mouse, Rat |
1: 500-1: 2500 |
brain, kidney, lung, skeletal muscle |
Background
RB1 belongs to the retinoblastoma protein family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1/H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by Smarxa4/Brg1 by recruiting a histone deacetylase complex to the c-FOS promoter. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Images
|
|
Immunohistochemistry analysis of paraffin-embedded mouse brain using RB-1 (GB111111) at dilution of 1: 1400 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat brain using RB-1 (GB111111) at dilution of 1: 2000 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat kidney using RB-1 (GB111111) at dilution of 1: 2000 |
|
|
Immunohistochemistry analysis of paraffin-embedded rat lung using RB-1 (GB111111) at dilution of 1: 1400 |
|
|
Immunohistochemistry analysis of paraffin-embedded mouse skeletal muscle using RB-1 (GB111111) at dilution of 1: 1000 |
|
|
Immunofluorescent analysis of paraffin-embedded mouse brain using RB-1 (GB111111) at dilution of 1: 1000 |
|
|
Immunofluorescent analysis of paraffin-embedded rat brain using RB-1 (GB111111) at dilution of 1: 1000 |
|
|
Immunofluorescent analysis of paraffin-embedded rat kidney using RB-1 (GB111111) at dilution of 1: 1000 |
|
|
Immunofluorescent analysis of paraffin-embedded rat lung using RB-1 (GB111111) at dilution of 1: 1000 |
|
|
Immunofluorescent analysis of paraffin-embedded mouse skeletal muscle using RB-1 (GB111111) at dilution of 1: 1000 |
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
凡合肥萬物生物科技有限公司銷售的產(chǎn)品均有嚴(yán)格的質(zhì)量保證。如發(fā)現(xiàn)產(chǎn)品存在包裝規(guī)格疑問，請(qǐng)于收貨之日起七日內(nèi)向我公司總部或當(dāng)?shù)剞k事處提出，并請(qǐng)保證該產(chǎn)品，以備本公司進(jìn)行核對(duì)檢驗(yàn)。如果發(fā)現(xiàn)有質(zhì)量問題，請(qǐng)于收貨后一月內(nèi)保留產(chǎn)品50%以上的量，以便本公司回收產(chǎn)品進(jìn)行質(zhì)檢，確認(rèn)產(chǎn)品質(zhì)量。如逾期，則視為已經(jīng)對(duì)本公司產(chǎn)品進(jìn)行驗(yàn)收。 聲明：如發(fā)生產(chǎn)品索賠事宜，本公司僅在產(chǎn)品價(jià)格范圍內(nèi)酌情賠償，恕不接受超出產(chǎn)品價(jià)格范圍之賠償。客戶在使用過程中有任何技術(shù)問題可以撥打技術(shù)售后服務(wù)電話我們隨時(shí)給予解答。
合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區(qū)黃山路602號(hào)合肥國(guó)家大學(xué)科技園A401室