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Anti -Histone H4 Rabbit pAb

價(jià)格: 在線咨詢
貨號(hào):
KT012196
產(chǎn)品規(guī)格:
Applications:
WB/IHC/ICC/IF
Species:
H/M/R,H/M/R,H/M/R
購(gòu)買數(shù)量:
產(chǎn)品詳情 操作說(shuō)明 注意事項(xiàng)

Product Information


Description

Histone H4 rabbit polyclonal

Protein full name

Histone H4

Synonyms

H4C1, H4/A, H4C2, H4C3, H4C4, H4/I, HIST1H4BAND, HIST1H4CAND, HIST1H4E

AND, HIST4H4, H4C12, HIST2H4, H4FN

Immunogen

KLH conjugated Synthetic peptide corresponding to Human Histone H4

Isotype

IgG

Purity

Affinity purification

Subcellular location

Nucleus

Predicted MW.

11 kDa

Observed MW.

11 kDa

Uniprot ID

P62805, P62806




Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 500-1: 1000

liver, lung, testis

IHC

Human, Mouse, Rat

1: 500-1: 1000

tonsil, lung, liver, colon, ovary, esophagus, testis, kidney, small intestine

ICC/IF

Human, Mouse, Rat

1: 100-1: 500

Hela, NIH373



Background

Histone H4 is a 103 amino acid protein, which belongs to the histone H4 family. Histone H4 localizes in the nucleus and is a core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. remodeling.




Images



Western blot analysis of Histone H4 (GB111688)

at dilution of 1: 1000

Lane 1: Hela cell lysate

Lane 2: C2C12 cell lysate

Lane 3: NIH3T3 cell lysate

Lane 4: Mouse liver tissue lysate

Lane 5: Mouse lung tissue lysate

Lane 6: Mouse testis tissue lysate

Lane 7: Rat liver tissue lysate

Lane 8: Rat testis tissue lysate

Immunohistochemistry analysis of paraffin-embedded human tonsil using

Histone H4 (GB111688) at dilution of 1: 1000


Immunohistochemistry analysis of paraffin-embedded human lung using

Histone H4 (GB111688) at dilution of 1: 1000


Immunohistochemistry analysis of paraffin-embedded human liver using

Histone H4 (GB111688) at dilution of 1: 1000


Immunohistochemistry analysis of paraffin-embedded human colon using

Histone H4 (GB111688) at dilution of 1: 1000



Immunohistochemistry analysis of paraffin-embedded rat colon using

Histone H4 (GB111688) at dilution of 1: 1000


Immunohistochemistry analysis of paraffin-embedded mouse colon using

Histone H4 (GB111688) at dilution of 1: 1000


Immunohistochemistry analysis of paraffin-embedded human ovary using

Histone H4 (GB111688) at dilution of 1: 1000


Immunohistochemistry analysis of paraffin-embedded human esophagus using

Histone H4 (GB111688) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded rat kidney using

Histone H4 (GB111688) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded rat kidney using

Histone H4 (GB111688) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded rat testis using

Histone H4 (GB111688) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded mouse testis using

Histone H4 (GB111688) at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded mouse small intestine using

Histone H4 (GB111688) at dilution of 1: 1000

A

B

Immunocytochemistry analysis of 4% paraformaldehyde-fixed Hela cell using Histone H4 (GB111688) at dilution of 1: 500

A: Hela cell-treated with green light and cyclopeptide.

B: Hela cell-treated with red light, green light and cyclopeptide.

A

B

Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH373 cell using Histone H4(GB111688) at dilution of 1: 500

A: NIH373 cell-treated with green light and cyclopeptide

B: NIH373 cell-treated with red light, green light and cyclopeptide.




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer (G2025).

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