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Anti -Synaptophysin Rabbit pAb

價格: 在線咨詢
貨號:
KT011767
產(chǎn)品規(guī)格:
100ul
Applications:
WB/IHC/IF
Species:
H/M/R,H/M/R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

Synaptophysin rabbit polyclonal antibody

Protein full name

Synaptophysin

Synonyms

SYP, MRX96, MRXsynaptophysin, MRXSYP, Major Synaptic Vesicle Protein P38

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse Synaptophysin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Synaptic vesicle membrane

Predicted MW.

34 kDa

Observed MW.

38 kDa

Uniprot ID

P08247, Q62277, P07825



Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1:5000-1:10000

brain, cerebral cortex, cerebellum

IHC/IF

Human, Mouse, Rat

1:1000-1:2000

brain, cerebellum, epencephalon, spinal cord




Background


Synaptophysin, also known as the major synaptic vesicle protein p38, is a protein that in humans is encoded by the SYP gene. The protein is a synaptic vesicle glycoprotein with four transmembrane domains weighing 38kDa. It is present in neuroendocrine cells and in virtually all neurons in the brain and spinal cord that participate in synaptic transmission. It acts as a marker for neuroendocrine tumors, and its ubiquity at the synapse has led to the use of synaptophysin immunostaining for quantification of synapses.

           



Images



Picture 1.png

Western blot analysis of Synaptophysin (GB11553) at dilution of 1: 5000

Picture 2.png Immunohistochemistry of paraffin embedded mouse brain using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Picture 3.png Immunohistochemistry of paraffin embedded mouse cerebellum using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Picture 4.png

Immunohistochemistry of paraffin embedded mouse spinal cord using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)

Picture 5.png Immunohistochemistry of paraffin embedded rat brain using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Picture 6.png Immunohistochemistry of paraffin embedded rat cerebellum using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)
Picture 7.png

Immunohistochemistry of paraffin embedded rat spinal cord using Synaptophysin (GB11553) at dilution of 1: 1000 (400x lens)

Picture 8.png

Immunofluorescence of paraffin embedded mouse brain using SYP (GB11553) at dilution of 1: 1000 (400x lens)

Picture 9.png

Immunofluorescence of paraffin embedded mouse epencephalon using SYP (GB11553) at dilution of 1: 1000 (400x lens)

Picture 10.png

Immunofluorescence of paraffin embedded mouse spinal cord using SYP (GB11553) at dilution of 1: 1000 (400x lens)
Picture 11.png Immunofluorescence of paraffin embedded rat brain using SYP (GB11553) at dilution of 1: 1000 (400x lens)

Picture 12.png

Immunofluorescence of paraffin embedded rat epencephalon using SYP (GB11553) at dilution of 1: 1000 (400x lens)




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


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