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Anti -GFAP Mouse mAb

價格: 在線咨詢
貨號:
KT011580
產(chǎn)品規(guī)格:
100 μL
Applications:
WB | IHC | IF
Species:
M,R/M,R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

GFAP mouse monoclonal antibody

Protein full name

Glial fibrillary acidic protein

Synonyms

GFAP, ALXDRD, glial fibrillary acidic protein

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse GFAP

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

49KDa

Observed MW.

49 kDa

Uniprot ID

P14136, P03995, P47819




Applications


Applications

Species

Dilution

Positive Sample

WB

Mouse, Rat

1:500-1:5000

brain, cerebral cortex, spinal cord

IHC/IF

Mouse, Rat

1:500-1:2000

brain



Background


Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells.GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. It is closely related to its non-epithelial family members, vimentin,desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton.




Images



Western blot analysis of GFAP (GB12096) at dilution of 1: 3000


Immunohistochemistry of paraffin embedded mouse brain using GFAP (GB12096) at dilution of 1: 600 (400x lens)


Immunohistochemistry of paraffin embedded rat brain using GFAP (GB12096) at dilution of 1: 600 (200x lens)


Immunofluorescence of paraffin embedded mouse brain using GFAP (GB12096) at dilution of 1: 1000 (400x lens)


Picture 5.png

Immunofluorescence of paraffin embedded rat brain using GFAP (GB12096) at dilution of 1: 1000 (200x lens)

Immunofluorescence of paraffin embedded rat brain using GFAP (GB12096) at dilution of 1: 1000 (400x lens)





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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